SEPSERV
HPLC

Separation Service Berlin
Helmholtzstr. 2 - 9, Aufg. L, D-10587 Berlin
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Tel.:  (030) 393 09 91    Fax: (030) 393 49 25  oder  391 01 000

Fruit Acids and Fatty Acids

UltraSep ESD FS

For the separation of small molecular acids, especially of fruit acids in food stuffs and similar materials, the packing UltraSep ES FS was developed. In the simplest case it is possible to use the method of ion suppression (Application FS1). If the mixtures of fruit acids cannot be separated satisfactory in this manner, individual solutions can be achieved by ion pairing chromatography (IPC; Application FS2). 

UltraSep ES WAX FS and UltraSep ES WAXI FS

For the case that special mixtures of mono- and di- or tricarbonic acids cannot be separated satisfactory by the methodical variants mentioned above, it is possible to determine the monocarbonic acids separately by the ion exchanger UltraSep ES WAX FS (Application FS3). Di- and tricarbonic acids will be removed from the column by back-flushing and, if necessary, separated by UltraSep ESD FS. 

The weak imino groups carrying ion exchanger UltraSep ES WAXI FS extends the selectivity of UltraSep ES WAX FS for monocarbonic acids within the pH range of 3.0 – 3.5 (Application FS4).

UltraSep ES FS

With this packing the separation of homologeous fatty acids by gradient elution is no problem (Application FS5). For the higher fatty acids we recommend UltraSep RP18 M500 (Application FS6).

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Analyzed in Application