Packing:

UltraSep ES SAX NN

Column:

60 x 3 mm

Detection:

UV 254 nm

Flow rate:

0.8  ml/min

Temperature:

30 °C 

Eluent A:

    3 mM NH₄H₂PO₄, pH 4.4

Eluent B:

200 mM NH₄H₂PO₄, pH 4.4

Temperature:

30 °C 

Gradient:

Start                 0% B
13.0th  min       7% B
13.1th min      28% B
20.2th min      29% B
20.3th min      53% B
28.0th min      85% B