|
Application
PROTEIN2 |
|
Packing:
|
UltraSep
ESD300 PROT RP18S
|
Column:
|
125
x 2 mm
|
Detection:
|
UV
220 nm
|
Eluent:
|
A:
10 mM Trifluoroacetic acid (TFA) in water
B: 9 mM TFA in acetonitrile/water (8/2)
|
Gradient:
|
Start
20% B
120th min 100 % B
140th min stop |
Flow
rate:
|
0.25
ml/min
|
Temperature:
|
36
°C
|
|
|
Proteins from Bacillus subtilis
with test compounds 1 - 4
at identical conditions |
|
|
1 |
Ribonuclease
(M = 13700) |
|
|
2 |
Cytochrome C
(M = 12400) |
|
|
3 |
Lysozyme
(M = 14400) |
|
|
4 |
Chymotrypsinogen
A
(M = 25000) |
|
|
|
|
|
| |
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