SEPSERV
HPLC

Separation Service Berlin
Helmholtzstr. 2 - 9, Aufg. L, D-10587 Berlin
Anfahrt/Approach

Tel.:  (030) 393 09 91    Fax: (030) 393 49 25  oder  391 01 000

E-Mail: sepserv.berlin@t-online.de  Internet: www.sepserv.de

 
Application UltraSep ES C30
Packing: UltraSep ES C30 DM, 5 µm

Column:

250 x 2 mm

Detection:

Peak 1/2 UV 462/430 nm
Peak 3/4 Fluorescence Ex 435/409 nm
                                      Em 662/674 nm

Gradient:

50 - 100% B

Eluent A:
Eluent B:

Org.-aqueous, buffered;
Org. tern.

Flow rate:

0.3 ml/min

Temperature:

35 °C

Chromatogram of a customer
Helmholtzzentrum Magdeburg

 

1

Chlorophyll b

2

Chlorophyll a

3

Phaeophytin b

4

Phaeophytin a

 

 

 

 

 

 

 

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