SEPSERV
HPLC

Separation Service Berlin
Helmholtzstr. 2 - 9, Aufg. L, D-10587 Berlin
Anfahrt/Approach

Tel.:  (030) 393 09 91    Fax: (030) 393 49 25  oder  391 01 000

Polycyclic Aromatic Hydrocarbons (PAH)

UltraSep ES PAH

Polycyclic aromatic hydrocarbons are very widespread pollutions in environment, and many laboratories analyze them.

10 years after introduction, our PAH columns are still top products on the market.  They reveal simple separation conditions, an excellent resolution (base line separation) of all compounds listed by EPA and DIN  as well as short times of analysis. The separation succeeds already with a simple water/acetonitrile gradient. Therefore, combined gradients or even ternary gradients are not necessary. Furthermore, a perfect wave length switching (fluorescence detection) is possible. Internal standards (e. g. Anthracene-d₁₀, Dibenz(a,h)anthracene-d₁₄ or 2-Methylchrysene can be positioned within the chromatogram without difficulties.

The often tested columns yield very reproducible results with constant retention times for more than 3000 injections. They work without problems in the temperature range of 20 – 40 °C.

in the EU according to the European Food Safety Authority (EFSA) the PAHs of the SCF (Scientific Committee on Food) list have to be analyzed in food, additionally to the PAHs of the EPA (Environmental Protection Agency, USA) list (see application PAHEFSA).

Using precolumns a contamination of the analytical columns by the samples can be avoided. We recommend our PAH Precolumn Cartridges.

Take into account, please, that 1 mm columns can be used effectively only, if the volume of the detector is below 1 µl. For optimal gradient separations not only the increase of the gradient, but also the adjusted volume flow is of importance. We have optimized the separation conditions for you. The parameters specified in the reference chromatograms are related to high pressure gradients. If you are working with a low pressure gradient,  the conditions have to be adapted because of the greater dwell time.

In the case that you can only work isocratically, keep in mind the large requirements of analysis time if higher aromatics are present. We recommend for this case a step gradient, which allows the separation of 2 x 8 = 16 aromatics within approximately 30 minutes. (I) Acetonitrile/water = 55/45, (II)  Acetonitrile/water = 90/10.

UltraSep ES PAH Quick Columns

The top quality packing UltraSep ES PAH is available also with a particle diameter of 4 µm. Because of that the separation columns reach an effectivity of >100000 TP/m and the duration of analysis can be shortened to one third without loss of quality. One advantage of the Quick Columns is a rapid adjustment of the equilibrium after each analysis. Therefore, chromatographical runs can follow immediately after each other.

Analyzed in Application

Acenaphthene

Acenaphthylene

Anthracene

Benz(a)anthracene

Benzo(b)fluoranthene

Benzo(k)fluoranthene

Benzo(g,h,i)perylene

Benzo(a)pyrene

Benzo(e)pyrene

Chrysene

Fluorene

Indeno(1,2,3-c,d)pyrene

2-Methylchrysene

1-Methylnaphthalene

2-Methylnaphthalene

Naphthalene

Phenanthrene

Pyrene